Integrative model of genomic factors for determining binding site selection by estrogen receptor alpha in MCF-7 cancer cells

ABSTRACT: Using the estrogen receptor alpha (ERalpha) as a model ligand inducible transcription factor, we sought to explicitly define parameters that determine transcription factor binding site selection on a genomic scale in an inducible system that minimizes confounding chromatin effects by the transcription factor itself. By examining several genetic and epigenetic parameters, we find that an energetically favorable estrogen response element (ERE) motif sequence, evidence of occupancy of a "pioneering" transcription factor FOXA1, the presence of the enhancer mark, H3K4me1, and an open chromatin configuration (FAIRE) at the pre-ligand state provide specificity for ER binding. Genome-wide ChIP-sequencing was done in MCF-7 cancer cell line for the following histone H3 modifications: monom

ORGANISM(S): Homo sapiens

SUBMITTER: Guoliang Li 

PROVIDER: E-GEOD-26831 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress