Gene expression response to the antifungal compound plakortide F acid in S. cerevisiae

ABSTRACT: Plakortide F acid (PFA), a marine-derived polyketide endoperoxide, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. In the present study, transcriptional profiling coupled with mutant and biochemical analyses were conducted using the model organism Saccharomyces cerevisiae, to investigate the mechanism of action of this compound. PFA elicited a transcriptome response indicative of a Ca2+ imbalance, affecting the expression of genes known to be responsive to altered cellular calcium levels. Several additional lines of evidence obtained supported a role for Ca2+ in PFA's activity. First, mutants lacking calcineurin and various Ca2+ transporters including pumps (Pmr1 and Pmc1) and channels

ORGANISM(S): Saccharomyces cerevisiae S288c

SUBMITTER: Ameeta Agarwal 

PROVIDER: E-GEOD-26925 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress