Expression data from REST knock-out versus REST wild type cells during in vitro neurogenesis

ABSTRACT: While changes in chromatin are integral to transcriptional reprogramming during cellular differentiation, it is currently unclear how chromatin modifications are targeted to specific loci. We developed a computational model on the premise that transcription factors (TFs) direct dynamic chromatin changes during cell fate decisions. When applied to a neurogenesis paradigm, this approach predicted the TF REST as a determinant of gain of Polycomb-mediated H3K27me3 in neuronal progenitor cells. We prove this prediction experimentally by showing that the absence of REST causes loss of H3K27me3 at target promoters in trans at the same cellular state. Moreover, promoter fragments containing a REST binding site are sufficient to recruit H3K27me3 in cis, while deletion of their REST site results in

ORGANISM(S): Mus musculus

SUBMITTER: Dirk Schuebeler 

PROVIDER: E-GEOD-27114 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress