Merging candidate gene approach with high throughput systems biology technology for the development of xylose utilising S. cerevisiae strains

ABSTRACT: Though highly efficient at fermenting hexose sugars, the ethanologenic yeast Saccharomyces cerevisiae has limited ability to ferment five-carbon sugars. As a significant portion of sugars found in cellulosic biomasses is the five carbon sugar xylose, S. cerevisiae must be engineered to metabolize pentose sugars. Here we combined classical candidate gene approach with systems biology to develop xylose-utilising S. cerevisiae strains. The introduction of an exogenous xylose isomerase (XYLA) and an additional copy of the endogenous xylulokinase gene (XKS1) results in the significant improvement of xylose consumption. Microarray studies reveal that the introduction of XYLA and XKS1 results in the dramatic transcriptional remodelling of the cell under both glucose and xylose conditions. To furt

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Linda Harris 

PROVIDER: E-GEOD-27325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress