Genomic differences distinguish the myofibroblast phenotype of distal lung from airway fibroblasts

ABSTRACT: ABSTRACT Primary human distal lung/parenchymal fibroblasts (DLF) exhibit a different phenotype from airway fibroblasts (AF), including the expression of high levels of a-smooth muscle actin (a-SMA). The scope of the differences and the mechanisms driving them are unknown. To determine whether distinct fibroblast characteristics and function based on lung region are predicted by a broad range of genomic differences in AF vs DLF. Matched human fibroblast pairs isolated from proximal and distal lung in 18 asthmatic and 4 normal subjects were studied. Microarray analysis was performed on 12 matched fibroblast pairs (8 asthmatic and 4 normal subjects) and validated by quantitative real-time PCR (qRT-PCR). The functional impact of these molecular differences on AF and DLF was then revealed usin

ORGANISM(S): Homo sapiens

SUBMITTER: XIUXIA ZHOU 

PROVIDER: E-GEOD-27335 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress