Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from WT, HEB-KO and E2A-KO LY6D- CLP cells


ABSTRACT: The E-protein transcription factors E2A and HEB play important roles at several stages of hematopoiesis. However, the exact mechanism for theire action and the main targets in the LY6D negative common lymphoid progentior (CLP) compartment remains unknown. By adressing this question, we will gain important infromation regarding the early events leading to B-cell specification. FACS sorted LY6D negative common lymphoid progenitors from WT, HEB-KO and E2A-KO mice were subjected to RNA extraction and hybridization on Affymetrix microarrays. At least two independent sorts were performed per genotype. During each sort, cells were pooled from several bone marrows.

ORGANISM(S): Mus musculus

SUBMITTER: Eva Welinder 

PROVIDER: E-GEOD-27402 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The transcription factors E2A and HEB act in concert to induce the expression of FOXO1 in the common lymphoid progenitor.

Welinder Eva E   Mansson Robert R   Mercer Elinore M EM   Bryder David D   Sigvardsson Mikael M   Murre Cornelis C  

Proceedings of the National Academy of Sciences of the United States of America 20111004 42


Recent studies have identified a number of transcriptional regulators, including E proteins, EBF1, FOXO1, and PAX5, that act together to orchestrate the B-cell fate. However, it still remains unclear as to how they are linked at the earliest stages of B-cell development. Here, we show that lymphocyte development in HEB-ablated mice exhibits a partial developmental arrest, whereas B-cell development in E2A(+/-)HEB(-/-) mice is completely blocked at the LY6D(-) common lymphoid progenitor stage. We  ...[more]

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