Microarray evidence for off target effects and the optimisation of targeted RNAi mediated gene silencing in the cattle tick Rhipicephalus (Boophilus) microplus

ABSTRACT: The NimbleGen cattle tick custom microarray based on the BmiGI.V2 database of R. microplus ESTs was used to screen the resulting mRNA harvested from ticks treated with the tick Ubiquitin-63E 600bp dsRNA in comparison to controls. A total of 144 ESTs including TC6372 (Ubiquitin-63E) were down-regulated with 136 ESTs up-regulated following treatment. The results obtained substantiated the knockdown phenotype with ESTs identified as associated with ubiquitin proteolysis as well as oogenesis, embryogenesis, fatty acid synthesis and stress responses. A stringent bioinformatics analysis was undertaken to predict off target effects (OTE) resulting from the in silico dicing of the 600bp Ubiquitin-63E dsRNA which identified 10 down (including TC6372) and 13 up-regulated ESTs within the list of dif

ORGANISM(S): Rhipicephalus microplus

SUBMITTER: Ala Lew-Tabor 

PROVIDER: E-GEOD-27453 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress