The Paradigm of Macrophage Polarization is not Applicable per seto Human Circulating Monocytes

ABSTRACT: Macrophage activation is required for the control of innate and adaptive immune responses. The classification in M1 and M2 macrophages based on a combination of small numbers of membrane and soluble markers is operational in murine and human macrophages. If this classification may be extended to circulating monocytes is not elucidated. To answer such question, human monocytes were stimulated for 6 and 18 hours with IFN-gamma and IL-4, two canonical agonists of M1/M2 polarization in macrophages, and gene expression programs were investigated with whole genome microarrays. The temporal analysis of these programs showed marked differences to both IFN-gamma and IL-4. In 6-h stimulated monocytes, gene categories related to inflammatory and immune responses were enriched, and these monocytes exh

ORGANISM(S): Homo sapiens

SUBMITTER: adil ef 

PROVIDER: E-GEOD-27658 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress