MiR-371-3 expression predicts neural differentiation potential in human pluripotent stem cells

ABSTRACT: The use of pluripotent stem cells in regenerative medicine and disease modeling is complicated by the variation in differentiation properties between lines. In this study, we characterized 13 human embryonic stem cell. (hESC) and 26 human induced pluripotent stem cell (hiPSC) lines to identify markers that predict neural differentiation behavior. At a general level, markers previously known to distinguish mouse ESCs from epiblast stem cells (EpiSCs) correlated with neural differentiation behavior. More specifically, quantitative analysis of miR-371-3 expression prospectively identified hESC and hiPSC lines with differential neurogenic differentiation propensity and in vivo dopamine neuron engraftment potential. Transient KLF4 transduction increased miR-371-3 expression and altered neuroge

ORGANISM(S): Homo sapiens

SUBMITTER: Jeffrey Zhao 

PROVIDER: E-GEOD-27834 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress