Identification of the 5' end of the pig miRNA by the RAKE technology

ABSTRACT: We applied a new computational approach to predict specie-specific and conserved miRNAs, than experimentally confirmed by a modified RNA-primed Array-based Klenow Extension (RAKE) method. We identified 489 conserved and 1,178 pig-specific novel miRNAs increasing our tally of confirmed miRNAs to 1,667 novel miRNAs. In addition, RAKE allowed the identification of miRNA isoforms (isomiRs) that we demonstrated to be differentially expressed across tissues suggesting that subtle variability in isomiR expression is regulated and biologically meaningful. 12K microarray was hybridized with a pool of small RNAs from 20 different tissues (superior vena cava, adipose tissue, lung, spleen, stomach, liver, intestine, kidney, descending aorta, left atrium, left ventricle, skeletal muscle, pulmonary aort

ORGANISM(S): Sus scrofa

SUBMITTER: Gerolamo Lanfranchi 

PROVIDER: E-GEOD-28139 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress