MiRNA-cardiocirculatory

ABSTRACT: The short length of miRNAs results in a high dynamic range of melting temperatures and therefore impedes a proper selection of detection probes or optimized PCR primers. While miRNA microarrays allow for massive parallel and accurate relative measurement of all known miRNAs, they have so far been less useful as an assay for absolute quantification. Here we developed a new method based not only to the hybridization process that presents the limits before described, but integrating the hybridization to an enzymatic reaction. Moreover we introduced spike-in in the hybridization-enzymatic reaction allowing the quantification of miRNAs respect to them, canceling biases related to sequence, labeling, or hybridization. An alternative method for the absolute miRNA quantization was recently propose

ORGANISM(S): Sus scrofa

SUBMITTER: Cagnin Stefano 

PROVIDER: E-MTAB-1938 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress