Germline and embryo gene expression of wild-type vs. mutants in lin-54, a component of the C. elegans DRM complex

ABSTRACT: DRM is a conserved transcription factor complex that includes E2F/DP and pRB family proteins and plays important roles in development and cancer. Here we perform microarray expression profiling analysis of lin-54, a DNA-binding member of the DRM complex. To identify genes regulated by LIN-54 in soma and germline, we analyzed wild-type and lin-54 mutant C. elegans embryos and isolated germlines. We chose embryos because they consist primarily of somatic cells, at a developmental stage with both active cell divisions and dynamic developmental gene expression programs. Since lin-54 null animals are sterile, embryos were obtained from a strain carrying the partial loss-of-function allele lin-54(n2990). Germlines were dissected from lin-54(n3423) null adults that lack detectable transcript and

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Kirsten Hagstrom 

PROVIDER: E-GEOD-28494 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress