A five-species transcriptome array for oral mixed-biofilm studies

ABSTRACT: Investigation of whole genome gene expression levels of P. gingivalis W83, F. nucleatum DSMZ 25586, S. sanguinis SK36, A. actinomycetemcomitans HK1651, S. mutans UA159 in an 24 h old culture. Additionally, whole genome gene expression level changes of S. mutans UA159 biofilm cells after co-cultivation with S. mitis ATCC 11843 were compared to its single species biofilm growth after 24 h. Aim: Demonstration of the usefulness of a five-species gene expression array. Multiple probes per gene enabled identification of single inter-species cross-hybridizing probes. The deletion of such probes lead almost not to the deletion of the whole gene. This was investigated and confirmed by a two-species biofilm expression analysis: The here described array was used for the identification of genes of S.

ORGANISM(S): Porphyromonas gingivalis W83

SUBMITTER: Sylvio Redanz 

PROVIDER: E-GEOD-28841 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress