Dissecting the individual contribution of LuxS and autoinducer-2 activity on Streptococcus sanguinis SK36 biofilm formation by chemical, luxS-, and sahH-complementation

ABSTRACT: In a transcriptome based trail, we figured out that the deletion of luxS has a massive influence to cell growth and metabolism of Streptococcus sanguinis SK36. The biofilm defective luxS deletion mutant was comlemented by transgenic sahH from Pseudomonas aeruginosa. Thus 209 of 216 influenced genes of the luxS mutant compared to the isogenic wildype were restored in their expression (fold change of n M-bM-^IM-% 3; p M-bM-^IM-$ 0.05), including genes involved in cell division processes, stress response and catabolite control. Phenotypically, the reduced biofilm depth of S. sanguinis SR DELTAluxS was elevated to wild type level by the complementation with sahH. Neither the addition of physiological concentrated artificial autoinducer 2 (AI-2) to the culture of S. sanguinis SR DELTAluxS nor t

ORGANISM(S): Streptococcus sanguinis SK36

SUBMITTER: Sylvio Redanz 

PROVIDER: E-GEOD-37007 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress