Cervical carcinoma-associated fibroblasts are DNA diploid and do not show evidence for somatic genetic alterations

ABSTRACT: Cancer-associated fibroblasts (CAFs) have been recognized as important contributors to cancer development and progression. However, opposing evidence has been published whether CAFs, in addition to epigenetic, also undergo somatic genetic alterations and whether these changes contribute to carcinogenesis and tumour progression. We combined multiparameter DNA flow cytometry, flow-sorting and 6K SNP-arrays to study DNA aneuploidy, % S-phase, loss of heterozygosity (LOH) and copy number alterations (CNAs) to study somatic genetic alterations in cervical cancer-associated stromal cell fractions (n = 58) from formalin-fixed, paraffin-embedded (FFPE) samples. Tissue sections were examined for the presence of CAFs. Microsatellite analysis was used to study LOH. By flow cytometry we found no proof

ORGANISM(S): Homo sapiens

SUBMITTER: Jan Oosting 

PROVIDER: E-GEOD-29143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress