Transcription Initiation Platforms and GTF recruitment at tissue-specific enhancers and promoters

ABSTRACT: Recent works have shown that RNA Polymerase (Pol) II can be recruited to, and transcribe, distal regulatory regions. Here, we analyzed transcription initiation and elongation through genome-wide localization of Pol II, General Transcription Factors (GTFs) and active chromatin in developing T-cells. We show that Pol II and GTFs are recruited to known, highly T-cell-specific enhancers. We extend this observation for the first time to many new putative enhancers, a majority of which are transcribed with or without polyadenylation. Importantly, we also identify genomic features characterized by large Pol II/GTFs recruitment and Transcriptional Initiation Platforms (TIPs) at promoters, intergenic and intragenic regions. TIPs are characterized by variable widths (0.4 to 10 kb), and correlate wit

ORGANISM(S): Mus musculus

SUBMITTER: Romain Fenouil 

PROVIDER: E-GEOD-29362 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress