Splicing enhances recruitment of methyltransferase HYPB/Setd2 and methylation of histone H3 lysine 36

ABSTRACT: Several lines of recent evidence support a role for chromatin in splicing regulation. Here we show that splicing can also contribute to histone modification, which implies a bidirectional communication between epigenetics and RNA processing. Genome-wide analysis of histone methylation in human cell lines and mouse primary T cells reveals that intron-containing genes are preferentially marked with H3K36me3 relative to intronless genes. In intron-containing genes, H3K36me3 marking is proportional to transcriptional activity, whereas in intronless genes H3K36me3 is always detected at much lower levels. Furthermore, splicing inhibition impairs recruitment of H3K36 methyltransferase HYPB/Setd2 and reduces H3K36me3, whereas splicing activation has the opposite effect. Moreover, the increase of H

ORGANISM(S): Homo sapiens

SUBMITTER: Romain Fenouil 

PROVIDER: E-GEOD-30902 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress