C57BL/6N Mice on High Fat Diet Display Reduced Intestinal and Hepatic Cholesterol Levels despite a Plasma Hypercholesterolemia

ABSTRACT: Background Small intestine and liver greatly contribute to whole body lipid, cholesterol and phospholipid metabolism but to which extent cholesterol and phospholipid handling in these tissues is affected by high fat Western-style obesogenic diets remains to be defined. We therefore quantified cholesterol and phospholipid concentrations in intestine and liver and determined fecal neutral sterol and bile acid excretion in C57Bl/6N mice fed for 12 weeks either a cholesterol-free high carbohydrate control diet or a high fat diet containing 0.03 % (w/w) cholesterol. To identify underlying mechanisms of dietary adaptation in intestine and liver, changes in gene expression were assessed by microarray and qPCR profiling, respectively. Results Animals on high fat diet showed increased plasma chole

ORGANISM(S): Mus musculus

SUBMITTER: Charles Desmarchelier 

PROVIDER: E-GEOD-29748 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress