A human ESC model for MLL-AF4 reveals an impaired early human hemato-endothelial specification

ABSTRACT: The MLL-AF4 fusion gene is a hallmark genomic aberration in high-risk acute lymphoblastic leukemia in infants. Although it is well-established that MLL-AF4 arises pre-natally during human development, its effects on hematopoietic development in utero remains unexplored. We have created a human-specific in vitro system to study early hemato-endothelial development in MLL-AF4-expressing human embryonic stem cells (hESCs). Differentiation and functional studies as well as clonal analyses and gene expression profiling reveal that expression of MLL-AF4 in hESCs has a phenotypic, functional and gene expression impact. It enhances the specification of hemogenic precursors from hESCs and impairs further hematopoietic commitment of these precursors in favour of the endothelial cell fate. Similar t

ORGANISM(S): Homo sapiens

SUBMITTER: Gustavo Melen 

PROVIDER: E-GEOD-29869 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress