Mapping of meiotic double-strand break (DSB) sites and DSB factor association near repetitive DNA elements in S. cerevisiae

ABSTRACT: DNA double strand breaks (DSBs) in repetitive sequences are a potent source of genomic instability, due to the possibility of non-allelic homologous recombination (NAHR). Repetitive sequences are especially at risk during meiosis, when numerous programmed DSBs are introduced into the genome to initiate meiotic recombination 1. Within the budding yeast repetitive ribosomal (r)DNA array, meiotic DSB formation is prevented in part through Sir2-dependent heterochromatin 2,3. Here, we demonstrate that the edges of the rDNA array are exceptionally susceptible to meiotic DSBs, revealing an inherent heterogeneity within the rDNA array. We find that this localised DSB susceptibility necessitates a border-specific protection system consisting of the meiotic ATPase Pch2 and the origin recognition com

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andreas Hochwagen 

PROVIDER: E-GEOD-30073 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress