Transcription profiling of rat brain tissues from animals with varying levels of access to cocaine

ABSTRACT: By using high-density oligonucleotide arrays, we profiled gene expression in reward-related brain regions of rats that developed escalated cocaine intake after extended access to cocaine (6 h per day). Rats allowed restricted daily access to cocaine (only 1 h) that displayed a stable level of cocaine intake and cocaine naive rats were used for controls. Four analysis methods were compared: Affymetrix microarray suite 4 and microarray suite 5, which use perfect-match-minus-mismatch models, and dchip and rma, which use perfect-match-only models to generate expression values. Results were validated by RT-PCR in individual animals from an independent replication of the experiment. A small number of genes was associated with escalated cocaine intake (ESC genes). Unexpectedly, of the brain regio

ORGANISM(S): Rattus norvegicus

DISEASE(S): cocaine addiction

SUBMITTER: Lena Dina van der Stap 

PROVIDER: E-GEOD-3016 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress