Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling of prostate epithelial cells in response to tissue contextual changes

ABSTRACT: To conduct comparative transcriptomic analyses on normal or malignant prostate epithelial cells in response to tissue contextual changes, we cultured immortalized prostate epithelial cells or prostate cancer cells as cell monolayers or three-dimensional organoids and profiled their transcriptomes in respective culture contexts. RWPE-1 (immortalized prostate epithelial cells) and LNCaP cells (prostate cancer cells) were seeded on reconstituted basement membrane (rBM)-coated culture plastics or cultured within three-dimensional (3D) rBM gels. Total RNA samples were collected from cell monolayers or 3D cell clusters (formed at day 2) or acini or spheroids (formed at day 6) in the rBM culture, followed by global gene expression profiling.

ORGANISM(S): Homo sapiens

SUBMITTER: Jimmy Su

PROVIDER: E-GEOD-30304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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