Transcriptional effect of an off-target shRNA targeting nucleostemin in human glioblastoma-derived cancer stem cells

ABSTRACT: Glioblastomas (GBM) may contain a variable proportion of active cancer stem cells (CSCs) capable of self-renewal, of aggregating into CD133+ neurospheres, and to develop intracranial tumors that phenocopy the original ones. We hypothesized that nucleostemin may contribute to cancer stem cell biology as these cells share characteristics with normal stem cells. Here we report that nucleostemin is expressed in GBM-CSCs isolated from patient samples. The significance of its expression was addressed by targeting the corresponding mRNA using lentivirally transduced short hairpin RNA (shRNA). We found that an off-target nucleostemin RNAi (shRNA22) abolishes proliferation and induces apoptosis in GBM-CSCs. Furthermore, in the presence of shRNA22, GBM-CSCs failed to form neurospheres in vitro or gr

ORGANISM(S): Homo sapiens

SUBMITTER: Jon Gil-Ranedo 

PROVIDER: E-GEOD-30448 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress