Conditional elimination of c-Met signaling in the liver aggravates oxidative stress and cholestasis, and deregulates lipid metabolism, under a high cholesterol diet

ABSTRACT: Background: Genetic elimination of c-Met signaling in the liver has been shown to impact many cellular pathways involved in repair, regeneration, lipid homeostasis and redox balance. In this study, we analyzed a nutritional model of hepatic steatosis in Met fl/fl ; Alb-Cre +/- (MetKO) mice in a 129SV/C57BL/6 background fed a high cholesterol diet for 30 days. Methods: Liver injury was determined by histopathology and plasma enzymes, transcriptomic changes were examined using gene expression microarrays, and evaluation of key molecules involved in liver damage and lipid homestasis were evaluated by Western blot analysis. Results: We observed that in comparison with wild type mice subjected to the same diet, MetKO mice developed a strong liver lipid deposition, inflammatory infiltration an

ORGANISM(S): Mus musculus

SUBMITTER: Daekwan Seo 

PROVIDER: E-GEOD-30651 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress