A cost-effective method for double stranded cDNA synthesis for microarray analysis

ABSTRACT: DNA microarrays are two-dimensional arrangements of specific probes deposited on a substrate that have been widely used in gene expression analysis by measuring mRNA accumulation. The use of this type of microarrays involves the synthesis of cDNA, which has to be double stranded (ds) if the microarray probes are of the positive strand. We have used a custom-synthesized non-commercial NimbleGen microarray from melon to evaluate an alternative method of ds cDNA synthesis, which differs substantially in its economical cost relative to a widely recommended method. The results suggested that both methods produce cDNA representative of the melon transcriptome to a similar extent, indicating that the alternative technique provides a cheaper method of ds cDNA synthesis for microarray gene expressi

ORGANISM(S): Cucumis melo

SUBMITTER: Daniel Gonzalez-Ibeas 

PROVIDER: E-GEOD-30693 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress