The Bromodomain protein Brd4 Insulates Chromatin from DNA Damage Signaling through Acetyl-Lysine Binding

ABSTRACT: DNA damage activates a complex signaling network in cells that blocks cell cycle progression, recruits factors involved in DNA repair, and/or triggers programs that control senescence or programmed cell death. Alterations in chromatin structure are known to be important for the initiation and propagation of the DNA damage response, although the molecular details are unclear. We investigated the role of chromatin structure in the DNA damage response by monitoring multiple timedependent checkpoint signaling and response events with a high-content multiplex image-based RNAi screen of chromatin modifying and interacting genes. We discovered that Brd4, a double bromodomain-containing protein, functions as an endogenous inhibitor of DNA damage signaling by binding to acetylated histones at sites

ORGANISM(S): Homo sapiens

SUBMITTER: Charles Whittaker 

PROVIDER: E-GEOD-30700 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress