Oncogenic BRD4-NUT protein interactions

ABSTRACT: To investigate the mechanism that drives dramatic mistargeting of active chromatin in NUT midline carcinoma, we have identified protein interactions unique to the BRD4-NUT fusion oncoprotein compared to wild type BRD4. Using crosslinking, affinity purification, and mass spectrometry, we identified the EP300 acetyltransferase as uniquely associated with BRD4 through the NUT fusion in both NMC and non-NMC cell types. We also discovered ZNF532 among a small number of candidates associated with BRD4-NUT in NMC patient cells but not present in 293T cells.

OTHER RELATED OMICS DATASETS IN: PRJNA379616

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Barry Zee  

LAB HEAD: Mitzi I. Kuroda

PROVIDER: PXD005786 | Pride | 2017-04-05

REPOSITORIES: Pride