Genome-wide reductions in active chromatin, paused RNA Polymerase II and nucleosome turnover during heat shock

ABSTRACT: Heat shock rapidly induces expression of a small set of genes while globally repressing transcription, making it an attractive system for studying alterations in the chromatin landscape that accompany changes in gene regulation. We have characterized these changes using low-salt extraction of intact micrococcal nuclease (MNase)-treated Drosophila S2 cell nuclei to determine the active nucleosomal and subnucleosomal chromatin landscapes. The low-salt-soluble fraction corresponds to classical "active" chromatin and includes distinct size fractions of MNase-protected particles that can be precisely mapped by paired-end sequencing. After heat shock, the distribution of low-salt-soluble nucleosomes showed an overall reduction over gene bodies, consistent with down-regulation of transc

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jorja Henikoff 

PROVIDER: E-GEOD-30755 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress