Genes downstream of p53 mediating its effect on megakaryopoiesis

ABSTRACT: In order to examine genes whose expression is altered as a result of the knock-down (KD) of p53, we used gene expression microarray analysis based on the CHRF cell line, a human megakaryoblastic cell line, which upon PMA stimulation undergoes polyploidization, and extends proplatelet-like cytoplasmic protrusions combined with apoptosis. The CHRF cell line has been validated in our laboratory as a good model of in vitro megakaryopoiesis by comparison to hematopoietic stem cells undergoing Mk differentiation. As described, we have previously generated stable p53-KD CHRF cell lines by lentiviral delivery of microRNA-adapted-short hairpin sequences targeting p53 as well as a suitable scrambled-control cell line. For the microarray analysis we utilized the 4x44K whole human genome microarrays f

ORGANISM(S): Homo sapiens

SUBMITTER: Eleftherios Papoutsakis 

PROVIDER: E-GEOD-30984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress