Polysome-associated mRNA levels upon glucose repletion

ABSTRACT: Cell survival in changing environments requires appropriate regulation of gene expression, including post-transcriptional gene regulatory mechanisms. Based on reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P-bodies and can return to active translation. We present evidence contradicting the notion that this model is a widespread and general phenomenon. First, genome-wide measurements of mRNA abundance, translation, and ribosome occupancy following glucose withdrawal show that most mRNAs are lost from the cell coincident with their loss from polysomes. Second, only a very limited sub-population of translationally repressed transcripts, comprising fewer than 400 genes, can be reactivated for translation upon glucose

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Joshua Arribere 

PROVIDER: E-GEOD-31220 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress