A RNA-seq-based gene expression profiling of radiation-induced tumorigenic mammary epithelial cells

ABSTRACT: To understand tumorigenesis and cancer progression of mammary epithelium, we established a cell model combining over-expression of human telomerase reverse transcriptase gene (hTERT) and heavy-ion radiation from normal human mammary epithelial cells. We subsequently used RNA-seq method to acquire their transcriptomes from two characteristic cell lines, an immortal epithelial cell line (I_hMEC) and a tumorigenic epithelial cell line (T_hMEC), and to look for differentially expressed genes (DEGs) between immortalization and tumorigenicity. We identified 7,053 DEGs, of which 84 were not only highly expressed but also significantly regulated. We found that house-keeping (HK) genes and tissue-specific (TS) genes were regulated differently during tumorigenesis; HK genes tend to be activated but

ORGANISM(S): Homo sapiens

SUBMITTER: lina ma 

PROVIDER: E-GEOD-31310 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress