Laboratory evolution of Jen1p-independent lactate transport in Saccharomyces cerevisiae: identification of ADY2 alleles by whole genome resequencing and mRNA expression analysis

ABSTRACT: Background: Evolutionary engineering is a powerful approach to isolate suppressor mutants and industrially relevant genotypes. Until recently, DNA microarray analysis was the only affordable genome-wide approach to identify the responsible mutations. This situation has changed due to the rapidly decreasing costs of whole genome (re)sequencing. DNA microarray-based mRNA expression analysis and whole genome resequencing were combined in a study on lactate transport in Saccharomyces cerevisiae. Jen1p is the only S. cerevisiae lactate transporter reported in literature. To identify alternative lactate transporters, a jen1Δ strain was evolved for growth on lactate. Results: Two independent evolution experiments yielded Jen1p-independent growth on lactate (μmax 0.14 and 0.18 h-1 for single-ce

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jean-Marc Daran 

PROVIDER: E-GEOD-31634 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress