Gene and pathways affected by CAG-repeat RNA-based toxicity in Drosophila

ABSTRACT: Spinocerebellar ataxia type 3 (SCA3) is one of the polyglutamine (polyQ) diseases, which are caused by a CAG repeat expansion within the coding region of the associated genes. The CAG repeat specifies glutamine, and the expanded polyQ domain with mutation confers dominant toxicity on the protein. Traditionally, studies have focused on protein toxicity in polyQ disease mechanisms. Recent findings, however, demonstrate that the CAG repeat RNA, which encodes the toxic polyQ protein, also contributes to the disease in Drosophila. To provide insight into the nature of the RNA toxicity, we extracted brain-enriched RNA from flies expressing a toxic CAG repeat mRNA (CAG100) and a non-toxic interrupted CAA/G mRNA repeat (CAA/G105) for microarray analysis. This approach identified a set of genes tha

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nancy Bonini 

PROVIDER: E-GEOD-31875 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress