Ontology highlight
ABSTRACT:
The 71Q and 105Q mice express a mutant version of the protein (TBP) and faithfully model the disease SCA17 (spinocerebellar ataxia-17, huntington disease-like 4, HDL4).
The constructs containing mixed CAG/CAA trinucleotide repeats (and encoding polyglutamine tracts) of variable length were made using a previously described method (Michalik A et al., Biotechniques, 2001). Briefly, synthetic CAG/CAA oligonucleotides were subcloned into a cDNA construct of the normal mouse (13 CAG/CAA) TBP gene. Because of the mixed nature of the repeat, its length is stable in mitotic and meiotic transmission.
ORGANISM(S): Mus musculus
SUBMITTER: Meyer Friedman
PROVIDER: E-MEXP-1313 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Friedman Meyer J MJ Shah Anjali G AG Fang Zhi-Hui ZH Ward Elizabeth G EG Warren Stephen T ST Li Shihua S Li Xiao-Jiang XJ
Nature neuroscience 20071111 12
Expansion of the polyglutamine (polyQ) tract in human TATA-box binding protein (TBP) causes the neurodegenerative disease spinocerebellar ataxia 17 (SCA17). It remains unclear how the polyQ tract regulates normal protein function and induces selective neuropathology in SCA17. We generated transgenic mice expressing polyQ-expanded TBP. These mice showed weight loss, progressive neurological symptoms and neurodegeneration before early death. Expanded polyQ tracts reduced TBP dimerization but enhan ...[more]