Transcription profiling of human native skin, cultured skin substitute, constituent keratinocytes and fibroblasts to identify gene signatures that are highly characteristic of different tissue types

ABSTRACT: Cultured skin substitutes, prepared using keratinocytes, fibroblasts and biopolymers, can facilitate closure of massive burn wounds by increasing the availability of autologous tissue for grafting. However, because they contain only two cell types, skin substitutes cannot replace all of the functions of native human skin. To better understand the physiological and molecular differences between cultured skin substitutes and native skin, we undertook a comprehensive analysis of gene expression in native skin, cultured keratinocytes, cultured fibroblasts, and skin substitutes using Affymetrix gene chip microarrays. Goals: Our analysis focused on identifying gene signatures that were highly characteristic of each cell and tissue type, and those that are regulated by the formation of cultured

ORGANISM(S): Homo sapiens

SUBMITTER: Bruce Aronow 

PROVIDER: E-GEOD-3204 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress