Bisulfite Sequencing Reveals that Aspergillus flavus Holds A Hollow in DNA Methylation

ABSTRACT: Aspergillus flavus first gained scientific attention for its production of aflatoxin, the most potent naturally occurring toxin and hepatocarcinogenic secondary metabolite. For several decades, The DNA methylation status of A. flavus remains to be controversial. We first applied bisulfite sequencing, the gold standard at present, in conjunction with a biological replicate strategy to investigate the DNA methylation profiling of A. flavus genome. Our results reveal that the DNA methylation level of this fungus turns out to be negligible, comparable to the unmethylated lambda DNA we set as the false positive control of our bisulfite experiments. When comparing the DNA methyltransferase homolog of A. flauvs with that from several selected hypermethylated speices, we find that the DNA methyltr

ORGANISM(S): Aspergillus flavus

SUBMITTER: Zhumei He 

PROVIDER: E-GEOD-32177 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress