Human and murine serum reactivity to specific histone posttranslational modifications in neutrophil extracellular traps

ABSTRACT: Introduction: Autoreactivity to histones is a pervasive feature of several human autoimmune disorders including systemic lupus erythematosus (SLE). Specific post-translational modifications (PTMs) of histones within neutrophil extracellular traps (NETs) may potentially drive the process by which tolerance to these chromatin-associated proteins is broken. We hypothesized that NETs and their unique histone PTMs might be capable of inducing autoantibodies that target histones. Methods: We developed a novel and efficient method for the in vitro production, visualization, and broad profiling of histone-PTMs of human and murine NETs. We also immunized Balb/c mice with murine NETs and profiled their sera on autoantigen and histone peptide microarrays for evidence of autoantibody production to

ORGANISM(S): Homo sapiens

SUBMITTER: Chih Long Liu 

PROVIDER: E-GEOD-32543 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress