Differentiation stage-specific donor memory in induced pluripotent stem cells (iPSC) generated from hepatic lineage cells

ABSTRACT: Recent studies suggested that embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) may represent different pluripotent states as defined by gene expression profiles and differentiation potential. Here we addressed a contribution of a lineage stage-specific donor cell memory in modulating the functional properties of iPSCs. iPSCs were generated from hepatic lineage cells at an early (hepatoblast-derived, HB-iPSCs) and end stage (adult hepatocyte, AH-iPSCs) of hepatocyte differentiation as well as from mouse fetal fibroblasts (MEF-iPSCs) using a lentiviral vector encoding four pluripotency-inducing factors Oct4, Sox2, Klf4, and c-Myc. All resulting iPS cell lines acquired iPSCs phenotype as judged by the accepted criteria including morphology, expression of pluripotency m

ORGANISM(S): Mus musculus

SUBMITTER: Daekwan Seo 

PROVIDER: E-GEOD-33110 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress