Identification of Oncogenic Potential of SIRT7 and Its Regulatory MicroRNAs in Liver Tumorigenesis

ABSTRACT: To investigate the specific roles of SIRT7 in the development of HCC, we employed large-scale gene expression analysis to identify the molecular signature that may affect enabling characteristics of cancer cells. Differentially expressed genes were analyzed on the Hep3B cells transfected with SIRT7 shRNAs, and recapitulated molecular signatures that related to hallmarks of cancer. For each of the experimental conditions, total RNA was extracted from three independent sets of the corresponding cell lines using TRIzol Reagent (Invitrogen), followed by clean up on Ambion columns (Illumina Total-Prep RNA Amplification Kit, Ambion). For each experimental condition, an RNA pool was then obtained by mixing equal quantities of total RNA from each of the three independent RNA extractions. Biotin-la

ORGANISM(S): Homo sapiens

SUBMITTER: JUNGWOO EUN 

PROVIDER: E-GEOD-33234 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress