Human glioblastoma cells: Tet-on(mutant p53 R175H expression) vs Tet-off(p53-null)

ABSTRACT: TP53 R175H mutation is one of the most common mutations in human cancers and cancer cells with this mutation stably express R175H protein in the nucleus. To identify the synthetic lethal gene interacted with the R175H, we conducted the high throughput screening by using tetracyclin inducible R175H expression system in SF126 cells and comprehensive shRNA library carried by lenti virus. We identified 906 candidate gene suppressions that may lead to accelerated cell growth inhibition under the presence of R175H. Inhibitor of differentiation 1 (ID1) was one of the candidate genes and suppression of ID1 by siRNA resulted in acceleration of growth inhibition of cell lines expressing endogenous R175H but not in TP53 null cell lines. The transient expression of R175H in TP53 null cell lines and su

ORGANISM(S): Homo sapiens

SUBMITTER: imai hiroo 

PROVIDER: E-GEOD-33362 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress