Chromatin position effects assayed by thousands of reporters integrated in parallel (tet-Off TRIP pools)

ABSTRACT: Reporter genes integrated into the genome are a powerful tool to reveal effects of regulatory elements and local chromatin context on gene expression. However, so far such reporter assays have been of low throughput. Here we describe a multiplexing approach for the parallel monitoring of transcriptional activity of thousands of randomly integrated reporters. More than 27,000 distinct reporter integrations in mouse embryonic stem cells, obtained with two different promoters, show ~1,000-fold variation in expression levels. Data analysis indicates that lamina-associated domains act as attenuators of transcription, likely by reducing access of transcription factors to binding sites. Furthermore, chromatin compaction is predictive of reporter activity. We also found evidence for cross-talk bet

ORGANISM(S): Mus musculus

SUBMITTER: Bas van Steensel 

PROVIDER: E-GEOD-49806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress