Architectural protein subclasses shape 3-D organization of genomes during lineage commitment

ABSTRACT: Understanding the topological configurations of chromatin can reveal valuable insights into how the genome and epigenome act in concert to control cell fate during development. Here we generate high-resolution architecture maps across seven genomic loci in embryonic stem cells and neural progenitor cells. We observe a hierarchy of 3-D interactions that undergo marked reorganization at the sub-Mb scale during differentiation. Distinct combinations of CTCF, Mediator, and cohesin show widespread enrichment in architecture at different length scales. CTCF/cohesin anchor long-range constitutive interactions that might form the topological basis for invariant sub-domains. Conversely, Mediator/cohesin together with pioneer factors bridge short-range enhancer-promoter interactions within and

ORGANISM(S): Mus musculus

SUBMITTER: Jennifer Phillips-Cremins 

PROVIDER: E-GEOD-36203 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress