Whole Genome Methylation Analysis of Metastatic Castration Resistant Prostate Cancer

ABSTRACT: Relatively little is known about how changes in gene copy number (CN) and gene CpG methylation interact to affect specific pathways in metastatic castration-resistant prostate cancer (CRPC). Oligonucleotide array comparative genomic hybridization (aCGH) was performed on DNA isolated from 15 metastatic CRPC samples. Commonly aberrant genes were evaluated in a confirmatory fashion using PCR, aCGH data from primary tumors, and existing CRPC expression data. Array-based comprehensive CpG methylation was assessed on the same sample set. A total of 495 genes (79 gained, 416 deleted) were CN aberrant in ?66% of the samples by aCGH, and 77 (9 amplified, 68 deleted) had statistically concordant expression including gain of AR and loss of PTEN and RB1. Significant CN differences were seen between th

ORGANISM(S): Homo sapiens

SUBMITTER: Terence Friedlander 

PROVIDER: E-GEOD-34174 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress