STAC3 is required for myotube formation and myogenic differentiation in vertebrate skeletal muscle

ABSTRACT: STAC3 was identified as a nutritionally regulated gene from an Atlantic salmon subtractive hybridization library with highest expression in skeletal muscle. Salmon STAC3 mRNA was highly correlated with myogenin and myoD1a expression during differentiation of a salmon primary myogenic culture and was regulated by amino acid availability. In zebrafish embryos, STAC3 was initially expressed in myotomal adaxial cells and in fast muscle fibres post-segmentation. Morpholino knockdown resulted in defects in myofibrillar protein assembly, particularly in slow muscle fibres, and decreased levels of the hedgehog receptor patched. The function of STAC3 was further characterized in vitro using the mammalian C2C12 myogenic cell line. STAC3 mRNA expression increased during the differentiation of the

ORGANISM(S): Mus musculus

SUBMITTER: Neil Bower 

PROVIDER: E-GEOD-34474 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress