High-throughput sequencing of AGO-immunoprecipitating miRs in human senescent fibroblast WI-38

ABSTRACT: In humans, there are four Ago proteins (Ago1M-bM-^@M-^S4) and AGO1- and 2 were previously implicated in TGS induced by exogenous siRNAs and microRNAs (miRs) directed against gene promoter transcripts via promotion of changes in histone covalent modifications and DNA methylation. Not-with-standing, many mechanistic details of this process remain poorly defined in human cells, and very little is known about the identity of possible endogenous signals, which may drive this process in human cells. Given the evolutionary conserved role of siRNAs and AGO proteins in TGS and heterochromatin formation, we set out to analyse their possible involvement in senesence-associated repression of E2F target genes. To obtain a detailed picture of AGO-immunoprecipitating miRs (RIP) in senescent cells, we u

ORGANISM(S): Homo sapiens

SUBMITTER: Moussa BENHAMED 

PROVIDER: E-GEOD-34494 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress