Antibody profiling of smallpox vaccinees with/without a 'take' using custom vaccinia virus proteome microarrays

ABSTRACT: Successful vaccination against smallpox with conventional vaccinia virus is usually determined by the development of a vesicular skin lesion at the site of vaccinia inoculation, called a ‘take’. Although previous vaccination is known to be associated with attenuation of the take, the immunology that underlies a no-take in vaccinia-naïve individuals is not well understood. We hypothesized that antibody profiling of individuals before and after receiving vaccinia would reveal differences between takes and no-takes that may help better understand the phenomenon. Using vaccinia proteome microarrays and recombinant protein ELISAs we first examined the antibody response in vaccinia-naïve individuals that failed to take after receiving different doses of the replication competent smallpox

ORGANISM(S): Homo sapiens

SUBMITTER: David Davies 

PROVIDER: E-GEOD-34931 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress