Identification and characterization of potent, broad-spectrum antifungal compounds using a yeast reporter bioassay.

ABSTRACT: New antifungal drugs are urgently needed due to the currently limited selection, the emergence of drug resistance, and the toxicity of several commonly used drugs. To identify drug leads, we screened small molecules using a Saccharomyces reporter bioassay in which the yeast heterologously expresses Hik1, a group III hybrid histidine kinase (HHK) from Magnaporthe grisea. Group III HHKs are integral in fungal cell physiology, and highly conserved throughout this kingdom; they are absent in mammals, making them an attractive drug target. Our screen identified compounds 13 and 33, which showed robust activity against numerous fungal genera including Candida, Cryptococcus and molds such as Aspergillus and Rhizopus. Drug-resistant Candida from patients were also highly susceptible to compoun

ORGANISM(S): Candida albicans

SUBMITTER: Andre Nantel 

PROVIDER: E-GEOD-35105 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress