Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression in mice with conditional over-activation of hedgehog signaling in the ovary

ABSTRACT: The objective of the study was to determine the cause of anovulation in transgenic mice with conditional over-activation of the hedgehog signaling pathway in the ovary. In Amhr2(cre/+)-SmoM2 transgenic mice, a constitutively active version of the hedgehog signal transducer, smoothened (SMO), known as SMOM2, is expressed in the ovary following cre-mediated recombination. The hypothesis tested is that changes in the neonatal ovary caused by over-activation of hedgehog signaling leads to the life-long anovulation phenotype in transgenic mice. Microarrays were used to examine differences in gene expression in ovaries of two day old wild-type mice (Amhr2(+/+)-SmoM2) and transgenic mice (Amhr2(cre/+)-SmoM2) in which the hedgehog signaling pathway was conditionally over-activated in the ovary. Two microarrays were performed. RNA samples were prepared from ovaries of 8 Amhr2(+/+)-SmoM2 control mice and 8 Amhr2(cre/+)-SmoM2 mutant mice on day two of age. RNA quality was assessed by measurement of ribosomal RNA on an Agilent 2100 Bioanalyzer.

ORGANISM(S): Mus musculus

SUBMITTER: Susan Quirk

PROVIDER: E-GEOD-35123 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Overactivation of hedgehog signaling alters development of the ovarian vasculature in mice.

Ren Yi Y Cowan Robert G RG Migone Fernando F FF Quirk Susan M SM

Biology of reproduction 20120607 6

The hedgehog (HH) signaling pathway is critical for ovarian function in Drosophila, but its role in the mammalian ovary has not been defined. Previously, expression of a dominant active allele of the HH signal transducer protein smoothened (SMO) in Amhr2(cre/+)SmoM2 mice caused anovulation in association with a lack of smooth muscle in the theca of developing follicles. The current study examined events during the first 2 wk of life in Amhr2(cre/+)SmoM2 mice to gain insight into the cause of ano ...[more]

PMID: 22402963

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Project description:Primordial follicle assembly is a process that occurs in the embryonic or early post natal ovary in which oocyte nests break down to form individual primordial follicles. The size of this initial pool of primordial follicles in part determines the reproductive lifespan of the female. Connective tissue growth factor (CTGF) was identified as a potential regulatory candidate for this process in a previous microarray analysis of follicle development. The current study examines the effects of CTGF and associated transforming growth factor beta 1 (TGFbeta-1) on follicle assembly. Ovaries were removed from newborn rat pups and placed in an organ culture system for two days to measure the effect of these factors on follicle assembly. In addition, ovaries were cultured and treated for ten days to determine the potential of CTGF and TGFbeta-1 to manipulate the primordial follicle pool size over a longer developmental time period. The ovaries treated with CTGF for two days were found to have an increased proportion of assembled follicles. TGFbeta-1 had no effect on primordial follicle assembly and in combination with CTGF decreased oocyte number in the ovary after two days of culture. Over ten days of treatment only the combined treatment of CTGF and TGFbeta-1 was found to cause an increase in the proportion of assembled follicles. Interestingly, treatment with TGFbeta-1 alone resulted in fewer total oocytes in the ovary and decreased the primordial follicle pool size after ten days of culture. Observations indicate that CTGF alone or in combination with TGFbeta-1 stimulates primordial follicle assembly and TGFbeta-1 can decrease the primordial follicle pool size. CTGF was found to regulate the ovarian transcriptome during primordial follicle assembly and an integrative network of genes was identified. CTGF is one of the first growth factors shown to promote primordial follicle assembly, while TGFbeta-1 is one of the first factors shown to decrease the primordial follicle pool size. These observations suggest the possibility of manipulating primordial follicle pool size and influencing female reproductive lifespan. We used microarrays to determine genes expressed differentially between control and CTGF (connective tissue growth factor) treated P0 ovary RNA samples from 3 control groups are compared to 3 CTGF treated ovary groups

Dataset Information

Gene expression in mice with conditional over-activation of hedgehog signaling in the ovary

Publications

Overactivation of hedgehog signaling alters development of the ovarian vasculature in mice.

Similar Datasets

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