High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis

ABSTRACT: In Bacillus subtilis and its relatives carbon catabolite control, a mechanism enabling to reach maximal efficiency of carbon and energy sources metabolism, is achieved by the global regulator CcpA (carbon catabolite protein A). CcpA in a complex with HPr-Ser-P (seryl-phosphorylated form of histidine-containing protein, HPr) binds to operator sites called catabolite responsive elements, cre. Depending on the cre box position relative to the promoter, the CcpA/HPr-Ser-P complex can either act as a positive or a negative regulator. The cre boxes are highly degenerate semi-palindromes with a lowly conserved consensus sequence. So far, studies aimed at revealing how CcpA can bind such diverse sites were focused on the analysis of single cre boxes. In this study, a genome-wide analysis of cre si

ORGANISM(S): Bacillus subtilis

SUBMITTER: Bogumila Marciniak 

PROVIDER: E-GEOD-35154 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress