Sindbis virus induces the production of a novel class of endogenous siRNAs in Aedes aegypti mosquitoes

ABSTRACT: We examined the biogenesis of mRNA-derived endogenous short-interfering RNAs (endo-siRNAs) in the disease vector mosquito, Aedes aegypti. Under standard conditions, mRNA-derived endo-siRNAs were produced from the bidirectional transcription of tail-tail overlapping gene pairs. Upon infection with the alphavirus, Sindbis virus (SINV), another class of mRNA-derived endo-siRNAs was observed. Genes producing SINV-induced endo-siRNAs were not enriched for overlapping partners or nearby genes, but were enriched for transcripts with long 3'UTRs. Endo-siRNAs from this class derived uniformly from the entire length of the target transcript, and were found to regulate the transcript levels of the genes from which they were derived. Strand-specific qPCR experiments demonstrated that antisense strands

ORGANISM(S): Aedes aegypti

SUBMITTER: Zach Adelman 

PROVIDER: E-GEOD-35161 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress